Anti-inflammatory activity of citrus pectin on chicken monocytes' immune response

Pectin is a dietary fibre composed of galacturonic acid, primarily found in the citrus fruits' cell walls. Citrus pectin (CP) has demonstrated antioxidative, anticancer, and anti-inflammatory properties humans animals. In broilers, CP supplementation improves energy utilization nutrient digestibility, but limited information on its effects chicken immunity available so far. This study aimed to assess vitro impact monocytes' immune response. Cells were purified from whole blood healthy chickens incubated with increasing concentrations (0, 0.25, 0.5, 0.75, 1 mg/mL) determine working concentration. The different cells' apoptosis viability assessed by measuring caspase-3 -7 metabolic activity (MTT assay), respectively. had no dose-dependent effect monocyte viability.Then, (0.5 chemotaxis phagocytosis transwell migration fluorescein-labelled E. coli incorporation, inhibited both phagocytosis.These data demonstrate that exerts an immunomodulatory role monocytes, supporting integration nutrition strategies might be beneficial for animal's health.